Journal: BMC Cancer

Article Title: The expression of Hexokinase 2 and its hub genes are correlated with the prognosis in glioma

doi: 10.1186/s12885-022-10001-y

Figure Lengend Snippet: HK2 expression is positively correlated with the malignancy of human glioma. A The expression of HK2 in different cancers by using ONCOMINE database analysis. B The expression levels of HK2 in different cancer types from TCGA and GTEx datasets. C Compared with adjacent normal tissues, the expression of HK2 was much higher in LGG and GBM from GEPIA database. Box plots of differential expression of HK2 in different histologies of glioma. D The ROC curve analysis between patients and healthy controls to HK2. E The expression levels of HK2 expression in HEB, T98, and GBM1 cell lines. F-K The expression levels of HK2 were upregulated in different glioma subgroups (e.g., GBM, oligodendroglioma, anaplastic astrocytoma, and anaplastic oligoastrocytoma) from different datasets (e.g., TCGA, Bredel, Mural, Sun, and French datasets). Data shown as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The human glioma cell line T98 cells and the normal human brain astrocyte cell line HEB cells, were purchased from China Center for Type Culture Collection (Shanghai, China).

Techniques: Expressing, Quantitative Proteomics

Journal: BMC Cancer

Article Title: The expression of Hexokinase 2 and its hub genes are correlated with the prognosis in glioma

doi: 10.1186/s12885-022-10001-y

Figure Lengend Snippet: Knockdown of HK2 expression results in the suppression of tumour metastasis and VM Formation in glioma cells. A Representative images and quantification of the inhibition of HK2 expression in si-RNAs targeted HK2 (si-HK2–1 and si-HK2–2)-mediated HK2 knockdown in T98 and GBM1 cell lines. B - C The suppression of HK2 expression can inhibit tumour metastasis by using wound healing ( B ) and transwell ( C ) assays in T98 and GBM1 cell lines. D Tube formation assay shows HK2 knockdown could induce the inhibition of VM Formation in glioma cells. Bars, 200 μm. Data shown as mean ± SEM.* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: The human glioma cell line T98 cells and the normal human brain astrocyte cell line HEB cells, were purchased from China Center for Type Culture Collection (Shanghai, China).

Techniques: Knockdown, Expressing, Inhibition, Tube Formation Assay

Journal: BMC Cancer

Article Title: The expression of Hexokinase 2 and its hub genes are correlated with the prognosis in glioma

doi: 10.1186/s12885-022-10001-y

Figure Lengend Snippet: The correlation between HK2 expression and immune infiltration level in LGG and GBM. A Evaluation of the correlation between HK2 expression and immune infiltration by using the TIMER 2.0 database. B - E The correlation between the expression ( B - C ) or DNA methylation ( D - E ) of HK2 expression and immune infiltration in LGG and GBM specimens from the GSCA database. F The correlation between the expression of HK2 and immune score in gliomas. G The correlation between HK2 and the confirmed immune checkpoints in gliomas. H The mRNA levels of TNF-α, IL-2, IL-3, IL-4, and IL-12 in HK2-knockdown and control groups in T98 and GBM1 cell lines. Data are shown as mean ± SEM. * P < 0.05, ** P < 0.01, **** P < 0.0001, ns, not significant

Article Snippet: The human glioma cell line T98 cells and the normal human brain astrocyte cell line HEB cells, were purchased from China Center for Type Culture Collection (Shanghai, China).

Techniques: Expressing, DNA Methylation Assay, Knockdown, Control

Journal: BMC Cancer

Article Title: The expression of Hexokinase 2 and its hub genes are correlated with the prognosis in glioma

doi: 10.1186/s12885-022-10001-y

Figure Lengend Snippet: GO term, KEGG pathway, and GSEA enrichment analyses of HK2-related genes. A - B Heatmap of the top 50 positively and negatively related genes with HK2. C KEGG pathway enrichment analysis using the GSEA database. D - I Gene set enrichment plots of the NF-κB ( D ), complement and coagulation cascades ( E ), Toll-like receptor ( F ), NOD-like receptor ( G ), Th17 cell differentiation ( H ), and JAK-STAT ( I ) signalling pathways. J - M GO and KEGG enrichment analyses of HK2 positively or negatively related genes (Pearson’s rho ≥0.5, P < 0.05). N Expression levels of p-STAT3, STAT3, p-Akt, Akt in HK2-knockdown and control groups in T98 and GBM1 cell lines . Data shown as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: The human glioma cell line T98 cells and the normal human brain astrocyte cell line HEB cells, were purchased from China Center for Type Culture Collection (Shanghai, China).

Techniques: Coagulation, Cell Differentiation, Expressing, Knockdown, Control

Journal: Viruses

Article Title: Histone Deacetylase Inhibitor Panobinostat Benefits the Therapeutic Efficacy of Oncolytic Herpes Simplex Virus Combined with PD-1/PD-L1 Blocking in Glioma and Squamous Cell Carcinoma Models

doi: 10.3390/v14122796

Figure Lengend Snippet: Panobinostat treatment facilitated oHSV replication and tumor cytotoxicity in human squamous cell carcinoma SCC9 cells. Human squamous cell carcinoma SCC9 ( A , B ) and Tca8113 cells ( C , D ) were pretreated with panobinostat (1, 10, or 100 nM) for 14 h and then infected with T1012G (0.1 or 1 PFU/Cell) for another 48 h, respectively. The infected cell pellets were harvested at 48 hpi. The titration was measured by conventional plaque assay on Vero cells after three freeze–thaw cycles. The cytotoxicity activity was assessed using a CCK8 assay. SCC9 ( E ) and Tca8113 ( F ) cells were mock- or pretreated with panobinostat as above, following infection with T1012G for 48 h. The cell viability of the infected cells was measured with a CCK8 assay (mean ± SD). The assay was performed in triplicate. Differences between datasets were assessed by Student’s t -test (two-tailed) using GraphPad Prism software. * p < 0.05 and ** p < 0.01.

Article Snippet: A172, D54, and Tca8113 cells were purchased from JOINN Biologics, SCC9 cells were purchased from the BeNa Culture Collection, and CT2A cells were purchased from BLUEFBIO.

Techniques: Infection, Titration, Plaque Assay, Activity Assay, CCK-8 Assay, Two Tailed Test, Software

Journal: Viruses

Article Title: Histone Deacetylase Inhibitor Panobinostat Benefits the Therapeutic Efficacy of Oncolytic Herpes Simplex Virus Combined with PD-1/PD-L1 Blocking in Glioma and Squamous Cell Carcinoma Models

doi: 10.3390/v14122796

Figure Lengend Snippet: Downregulation of IFN-β- and IFN-stimulated antiviral genes when treated with panobinostat in human glioma and squamous cell carcinoma cells. A172 ( A1 , B1 , C1 , D1 ), D54 ( A2 , B2 , C2 , D2 ), SCC9 ( E1 , F1 , G1 , H1 ), or Tca8113 ( E2 , F2 , G2 , H2 ) cells were mock-treated or treated with panobinostat at different concentrations of 1, 10, or 100 nM for 14 h, respectively. Then, the cell pellets were harvested and total RNAs were extracted and reverse-transcribed to cDNA as described in the Materials and Methods section. The IFN-β, STAT1, PKR, and PML mRNAs were quantified and normalized with respect to 18S rRNA and are shown as fold changes compared to the mRNAs from the mock-treated and infected cells. Differences between datasets were assessed by Student’s t -test (two-tailed) using GraphPad Prism software. NS p >0.05, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: A172, D54, and Tca8113 cells were purchased from JOINN Biologics, SCC9 cells were purchased from the BeNa Culture Collection, and CT2A cells were purchased from BLUEFBIO.

Techniques: Reverse Transcription, Infection, Two Tailed Test, Software

Journal: Viruses

Article Title: Histone Deacetylase Inhibitor Panobinostat Benefits the Therapeutic Efficacy of Oncolytic Herpes Simplex Virus Combined with PD-1/PD-L1 Blocking in Glioma and Squamous Cell Carcinoma Models

doi: 10.3390/v14122796

Figure Lengend Snippet: Suppression of the transcriptional expression of cGAS/STING in human glioma and squamous cell carcinoma cells when treated with panobinostat. A172 ( A1 , B1 , C1 , D1 ), D54 ( A2 , B2 , C2 , D2 ), SCC9 ( E1 , F1 , G1 , H1 ), or Tca8113 ( E2 , F2 , G2 , H2 ) cells were mock-treated or treated with panobinostat at different concentrations of 1, 10, or 100 nM for 14 h, respectively. Then, the cell pellets were harvested and total RNAs were extracted and reverse-transcribed to cDNA as described in the Materials and Methods section. cGAS, STING, TBK1, and IRF3 were quantified and normalized with respect to 18S rRNA and shown as fold changes. Differences between datasets were assessed by Student’s t -test (two-tailed) using GraphPad Prism software. * p < 0.05, ** p < 0.01.

Article Snippet: A172, D54, and Tca8113 cells were purchased from JOINN Biologics, SCC9 cells were purchased from the BeNa Culture Collection, and CT2A cells were purchased from BLUEFBIO.

Techniques: Expressing, Reverse Transcription, Two Tailed Test, Software

Journal: Viruses

Article Title: Histone Deacetylase Inhibitor Panobinostat Benefits the Therapeutic Efficacy of Oncolytic Herpes Simplex Virus Combined with PD-1/PD-L1 Blocking in Glioma and Squamous Cell Carcinoma Models

doi: 10.3390/v14122796

Figure Lengend Snippet: Panobinostat treatment upregulated the PD-L1 expression in glioma and squamous cell carcinoma cells. A172 ( A , B ), SCC9 ( C , D ), D54 ( E ), Tca8113 ( F ), CT-2A ( G ), or SCC7 ( H ) cells were mock-treated or treated with panobinostat at different concentrations of 1, 10, or 100 nM for 14 h, respectively. Then, the cell pellets were harvested. For each condition, half of the cell pellets were used for total RNA extraction, following reverse transcription to cDNA. The other half of the cell pellets were lysed and used for Western blot (WB) as described in the Materials and Methods section. The assay was performed in triplicate. Differences between datasets were assessed by Student’s t -test (two-tailed) using GraphPad Prism software. * p < 0.05, ** p < 0.01.

Article Snippet: A172, D54, and Tca8113 cells were purchased from JOINN Biologics, SCC9 cells were purchased from the BeNa Culture Collection, and CT2A cells were purchased from BLUEFBIO.

Techniques: Expressing, RNA Extraction, Reverse Transcription, Western Blot, Two Tailed Test, Software